Research progress of CRISPR-based biosensors and bioassays for molecular diagnosis.

CRISPR/Cas technology originated from the immune mechanism of archaea and bacteria and was awarded the Nobel Prize in Chemistry in 2020 for its success in gene editing. Molecular diagnostics is highly valued globally for its development as a new generation of diagnostic technology. An increasing number of studies have shown that CRISPR/Cas technology can be integrated with biosensors and bioassays for molecular diagnostics. CRISPR-based detection has attracted much attention as highly specific and sensitive sensors with easily programmable and device-independent capabilities. The nucleic acid-based detection approach is one of the most sensitive and specific diagnostic methods. With further research, it holds promise for detecting other biomarkers such as small molecules and proteins. Therefore, it is worthwhile to explore the prospects of CRISPR technology in biosensing and summarize its application strategies in molecular diagnostics. This review provides a synopsis of CRISPR biosensing strategies and recent advances from nucleic acids to other non-nucleic small molecules or analytes such as proteins and presents the challenges and perspectives of CRISPR biosensors and bioassays.

Improving the safety and security of fruits and vegetables during COVID-19 pandemic with postharvest handling.

The COVID-19 pandemic has damaged the world's economy during 2020-2021, reduced the purchasing power of households, partially restricted international movements and trade (including food products) and damaged horticultural production. This resulted in uncertainty in the food business and caused food supply shocks. According to some scientists, business people, and politicians, this situation is a forerunner and warning for humanity to change its lifestyle by focusing on sustainable measures to prevent natural ecosystems damage. In line with this, the present review article focused on the significant impacts of the COVID-19 pandemic on horticultural production and some prevention measures. It has been scientifically confirmed that the postharvest losses of fruits and vegetables reach around 10-15% in developed countries and about 20-40% in developing countries, higher in some specific crops. It is believed that reducing these losses can help the world fight food supply shocks during the COVID-19 pandemic and customary conditions to reduce the pressure on natural resources. Therefore, the present paper aimed to highlight some critical handling practices against food supply shocks.